Museum collections are devoted to the history of shipping, boating and industry on the Hudson and its tributaries, such as the Rondout, where Kingston grew prosperous early in the 19th century. The city was the busiest port between New York City and Albany. 

The Museum is home to the Kingston High School Crew Team, the Rondout Rowing Club and the Kingston Sailing Club. The museum is a membership-supported organization and sponsors festivals and events including the Antique and Classic Boat Society Boat Show, Hudson River Days with music, crafts and displays, the "Follow the River" Lecture Series, Cinema Sundays and Family Days.